Loading Buffers and Dyes

Ammonium Persulfate

Synonyms:  Ammonium Peroxydisulfate.
Description: White crystalline powder.
Applications:  Used as an initiator in the polymerization of acrylamide.
Molecular Formula:  (NH₄)₂S₂O₈
Molecular Weight:  228.20.
CAS #   7727-54-0.

Purity.................................................... >98.0%
Melting point................... >120°C decomposes
Insolubles........................................... <0.005%
Residue after ignition............................ <0.05%
Solubility..................................................... 40%
Titratable free acid........................ <0.04 meq/g
Ash....................................................... <0.05%
Lead.................................................... <0.005%
Iron...................................................... <0.001%
Manganese.................................... <0.00005%
Chloride and chlorate......................... <0.001%
HAZARD.................................................... O, C
STORAGE................................................... RT

10mM ATP Solution

Synonyms:  Adenosine-5'-triphosphate.
Description:  Clear sterile solution in water, pH adjusted to 7.0.  Tested for use in ligation reactions.
Applications:  For use in molecular biology applications.
Molecular Formula:  C₁₀H₁₄O₁₃N₅P₃Na₂.3H₂O
Molecular Weight:  605.24.
CAS #  NA.

HAZARD...........................................................I
STORAGE...................................................... F

100mM ATP Solution, pH 7.5

Synonyms:  Adenosine-5'-triphosphate.
Description:  100mM ATP Solution. Clear sterile solution in water, pH adjusted to 7.5.  Tested for use in ligation reactions.
Applications:  For use in molecular biology applications.
Molecular Formula:  C₁₀H₁₄O₁₃N₅P₃Na₂.3H₂O
Molecular Weight:  605.24.
CAS #  NA

HAZARD...........................................................I
STORAGE...................................................... F

Boric Acid Ultra Pure Grade

Synonyms:  Boric acid, Borofax.
Description:  White crystalline powder, odorless.
Applications:  Used in buffers in conjunction with Tris for the preparation of agarose or polyacrylamide gels and their associated running buffers.
Molecular Formula:  H₃BO₃
Molecular Weight:   61.83.
CAS # :  10043-35-3.

Purity.................................................... >99.5%
Insolubles (methanol)......................... <0.005%
Non-volatiles (methanol)...................... <0.05%
Chloride.............................................. <0.001%
Phosphate.......................................... <0.001%
Sulfate.................................................. <0.01%
Arsenic............................................. <0.0001%
Calcium.............................................. <0.005%
Iron...................................................... <0.001%
Lead.................................................... <0.001%
HAZARD........................................................I,T
STORAGE................................................... RT

Coomassie Blue G-250 Stain

Description:  10% acetic acid, 0.025% coomassie blue
G-250.
Applications:  Used as a protein stain commonly used with polyacrylamide gels.
Shipping Conditions:  Ambient.
Storage:  Room temperature.

Coomassie Blue R-250 Stain

Description:  50% methanol, 0.05% coomassie brilliant blue R-250, 10% acetic acid.
Applications:  Used as a protein stain.  Used with            polyacrylamide gels.  A sensitive protein stain.
Shipping Conditions:  Ambient.
Storage:  Room temperature.

DNA Sequencing Dilution Buffer I

Description::  200mM Tris-HCl, pH 9.0, 5mM magnesium chloride, sterile solution.
Applications:  For Dilution of PE/Applied Biosystems^TM DNA Sequencing Reactions.
Shipping Conditions:  Ambient.
Storage:  Room temperature.

DNA Sequencing Dilution Buffer II

Description:  400mM Tris-HCl, pH 9.0, 1mM MgCl₂.
Applications:  For dilution of PE/Applied Biosystems^TM DNA sequencing reactions.
Shipping Conditions:  Ambient.
Storage:  Room temperature.

DNA Sequencing Dilution Buffer III

Description:  400mM Tris-HCl, pH 9.0, 10mM MgCl₂.
Applications:  For dilution of PE/Applied Biosystems^TM DNA sequencing reactions.
Shipping Conditions:  Ambient.
Storage:  Room temperature.

EDTA

Synonyms:  Ethylenediaminetetraacetic acid disodium dihydrate.
Description:  White crystalline odorless powder.
Applications:  Chelating agent.  Commonly used to inhibit enzymes that require divalent cations for activity.
Molecular Formula:  C₁₀H₁₄N₂O₈Na₂·2H₂O
Molecular Weight:  372.24.
CAS # :  6381-92-6.

Purity.................................................... >99.0%
Insolubles........................................... <0.005%
pH (5%, H₂O, 25°C)............................ 4.0 - 6.0
Lead.................................................... <0.005%
Iron........................................................ <0.01%
Nitrilotriacetic acid.................................. <0.1%
DNase, RNase, Protease......... None detected
HAZARD........................................................... I
STORAGE........................................................RT

Ethidium Bromide Solution

Description:  10 mg/ml ethidium bromide solution.
Applications:  Used to detect both single and double stranded nucleic acids.
Shipping Conditions:  Ambient.
Storage:  Room temperature.