Loading Buffers and Dyes (continued)

10 x Ficoll Loading Buffer

Description::  20% Ficoll, 100mM EDTA, 1.0% SDS, 0.25% bromophenol blue, 0.25% xylene cyanol.
Applications:  For agarose gels, DNA or RNA.
Shipping Conditions:  On ice.
Storage:  Room temperature.

100% Formamide Loading Buffer

Description:   10ml Deionized formamide. 
Applications:  For manual DNA sequencing gels.
Shipping Conditions:  Ambient.
Storage:  Freeze.

Formamide, deionized             

Synonyms:  Carbamaldehyde, methanamide.
Description:  Colorless, slightly viscous deionized liquid.
Applications:  Commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron ­­microscopy, and hybridization.
Molecular Formula:  CH₃NO
Molecular Weight:   45.04.
CAS #:  75-12-7.

Purity.................................................... >99.5%
Conductivity.................................. <100mmhos
A280......................................................... <0.05
Melting range........................................... 1-3°C
Boiling point (760mmHg).... 210° Decomposes
Vapor pressure (mm Hg).................. 1 @ 70°C
Iron.................................................. <0.00005%
Copper............................................ <0.00001%
Lead................................................ <0.00005%
Zinc................................................. <0.00005%
RNase, DNase, Protease......... None Detected
Hazard....................................................... I, ST
Storage........................................................... F

80% Formamide Loading Buffer

Description:  80% Formamide, 1.0mM EDTA, 45mM Tris base, 45mM boric acid, 0.05% bromophenol blue, 0.05% xylene cyanol.
Applications:  For use in analysis of messenger RNA and DNA sequencing.
Shipping Conditions:  Ambient.
Storage:  Freeze.

Formamide Stop/Loading Buffer II

Description:  98.0% Formamide, 10mM EDTA, 0.1% bromophenol blue, 0.1% xylene cyanol.
Applications:  For DNA sequencing and denaturing gels.
Shipping Conditions:  Ambient.
Storage:  Freeze.

10 x Glycerol Loading Buffer

Description::  60% Glycerol, 0.25% bromophenol blue, 0.25% xylene cyanol.
Applications:  For agarose gels, DNA or RNA
Shipping Conditions:  Ambient.
Storage:  Room temperature.

SDS, (Sodium Dodecyl Sulfate) Ultra Pure Grade

Synonyms:  Lauryl Sulfate Sodium Salt.
Description:  White, free flowing crystals or powder. 
SDS is a detergent that functions as a protein denaturant.
Applications:  Anionic detergent used to solublize proteins and cell membranes. Suitable for use in SDS-PAGE.
Molecular Formula:  C₁₂H₂₅NaO₄S
Molecular Weight:   288.38.
CAS #:  151-21-3.

Purity.................................................... >99.0%
A260 (3%, H₂O)......................................... <0.1
A280 (3%, H₂O)......................................... <0.1
Chloride.................................................. 0.05%
Phosphate........................................ <0.0005%
Lead.................................................. <0.0005%
Copper.............................................. <0.0005%
 DNase, RNase activity............. None detected
C12 Content......................................... >99.0%
Loss on Drying......................................... 1.0%
HAZARD........................................................... I
STORAGE................................................... RT

TEMED Ultra Pure Grade
(N,N,N', N'-Tetramethylethylenediamine)

Synonyms:  1,2-bis[dimethylamino]ethane, TMEDA.
Description:  Clear liquid when not oxidized.
Applications:  Used with ammonium persulfate as an initiator in the polymerization of acrylamide.
Molecular Formula:  C₆H₁₆N₂
Molecular Weight:   116.21.
CAS #:  110-18-9.

Purity.................................................... >99.0%
Melting point............................................. -55°C
EM (220nm , 2% Methanol)........................ >25
Boiling range.................................. 119 - 121°C
Refractive index........................... 1.417 - 1.419
Solubility (5% , H₂O)............................. Soluble
HAZARD..................................................... C, F
STORAGE................................................... RT

Tris Ultra Pure Grade
Tris-(hydroxymethyl)aminomethane

Synonyms:  THAM, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, tromethamine, trometamol.
Description:  Fine white crystals.
Applications:  Used in biological and electrophoresis buffers.
Molecular Formula:  C₄H₁₁NO₃
Molecular Weight:  121.14.
CAS #:  77-86-1.

Purity.................................................... >99.9%
Melting point............................................ 170°C
Moisture.................................................. <1.0%
Solubility (10% , H₂O).............. Clear/haze-free
Insolubles........................................... <0.005%
A280 (1.0M , H₂O)................................ <0.05%
Arsenic............................................. <0.0005%
Lead.................................................. <0.0001%
Iron.................................................... <0.0001%
Copper.............................................. <0.0001%
Magnesium....................................... <0.0005%
HAZARD........................................................... I
STORAGE................................................... RT

Urea

Synonyms:  Carbamide; carbonyl diamide.
Description:  White crystalline beads.
Applications:  Used to denature proteins and nucleic acids.  Used in electrophoresis for DNA sequencing.
Molecular Formula:  CH₄N₂O
Molecular Weight:   60.06.
CAS#:  57-13-6.

Purity.................................................... >99.5%
Melting point............................................ 132°C
Boiling point(760mmHg)............. Decomposes
Specific gravity(H10=1)........................... 1.335
Solubility in water.................................. Soluble
A280 (5M, H₂O).................................... <0.05%
Melting range................................. 132 - 135°C
Chloride.............................................. <0.007%
Lead.................................................... <0.002%
Sulfate.................................................. <0.01%
Identification.......................................... Passes
Residue on ignition................................. <0.1%
Insoluble (alcohol)................................ <0.04%
HAZARD........................................................... I
STORAGE................................................... RT