ANTIBIOTIC DATA

Ampicillin

Stock Conc.(mg/ml) : 50
Solvent : Water
Final Conc.(µg/ml) : 25-150
Description : Ampicillin and is recommended for use with all pUC derived plasmids carrying the beta-lactamase gene (e.g. pUC19, pBluescript, pGEM, etc...).
Expiration Date: 60 Days

Carbenicillin

Stock Conc.(mg/ml) : 25-150
Solvent : Water
Final Conc.(µg/ml) : 75
Description : Bacteriocidal. Carbenicillin is an analog of ampicillin and is recommended when using pUC derived plasmids such as pUC19, Bluescript, pGEM, etc... Carbenicillin demonstrates improved heat stability over ampicillin when used in growth media such as LB and Terrific Broth.
Expiration Date: under 100(mg/ml) = 45 Days, Over 100(mg/ml) = 60 Days

Chloramphenicol

Stock Conc.(mg/ml) : 10
Solvent : Methanol
Final Conc.(µg/ml) : 10-50
Description: Bacteriostatic; inhibits protein synthesis by interacting with the 50S ribosomal subunit and inhibiting the peptidyltransferase reaction. Chloramphenicol acetyltransferase inactivates chloramphenicol.
Expiration Date: = 135 Days

D-Cycloserine

Stock Conc.(mg/ml) : 10
Solvent : 0.1M Sodium phosphate buffer, pH 8.0
Final Conc.(µg/ml) : 200
Description : Bacteriocidal. D-Cycloserine only kills growing E.coli. It inhibits cell wall synthesis by preventing formation of D-alanine from L-alanine and formation of peptide bonds involving D-alanine. Mutations destroy the D-alanine transport system.

Gentamycin

Stock Conc.(mg/ml) : 10
Solvent : Water
Final Conc.(µg/ml) : 15
Description : Bacteriocidal. Gentamycin inhibits protein synthesis by binding to the L6 protein of the 50S ribosomal subunit. Aminoglycoside acetyltransferase and aminoglycosidenucleotidyltransferase inactivates gentamycin; mutations in rplF (encodes the L6 protein) prevents the gentamycin from binding.

Kanamycin

Stock Conc.(mg/ml) : 25
Solvent : Water
Final Conc.(µg/ml) : 25-50
Description: Bacteriocidal. Kanamycin inhibits protein synthesis, inhibits translocation and elicits miscoding. Aminoglycoside phosphotransferase, also known as neomycin phosphotransferase, aminoglycoside acetyltransferase, and aminoglycoside nucleotidyltransferase, inactivates kanamycin.
Expiration Date: = 150 Days

Nalidixic Acid

Solvent : Water, pH adjus to 11 with NaOH
Final Conc.(µg/ml) : 15
Description : Bacteriostatic. Inhibits DNA synthesis by inhibiting DNA gyrase. Mutations in the host DNA gyrase prevents nalidixic acid from binding.

Rifampicin

Stock Conc.(mg/ml) : 34
Solvent : Methanol
Final Conc.(µg/ml) : 150
Description : Bacteriostatic. Rifampicin inhibits RNA synthesis by binding to and inhibiting the â subunit or RNA polymerase; rifampicin sensitivity is dominant. Mutations in the â subunits of RNA polymerase prevents rifampicin from complexing; rifampicin resistance is recessive.

Spectinomycin

Stock Conc.(mg/ml) : 10
Solvent : Water
Final Conc.(µg/ml) : 100
Description : Bacteriostatic. Spectinomycin inhibits translocation of peptidyl tRNA from the A site to the P site. Mutations in rpsE (encodes the S5 protein) prevent spectinomycin sensitivity is dominant and resistance is recessive.
Expiration Date: = 90 Days

Streptomycin

Stock Conc.(mg/ml) : 50
Solvent : Water
Final Conc.(µg/ml) : 25-50
Description : Bacteriocidal. Inhibits protein synthesis by binding to the S12 protein of the 30S ribosomal subunit and inhibiting proper translation; streptomycin sensitivity is dominant.
Expiration Date: = 90 Days

Tetracycline

Stock Conc.(mg/ml) : 15
Solvent : 70% Ethanol
Final Conc.(µg/ml) : 12-75
Description: Bacteriostatic. Tetracycline inhibits protein synthesis by preventing binding of aminoacyl tRNA to the ribosome A site. Active efflux of drug from cell.
Expiration Date: = 90 Days

Trimethoprim

Stock Conc.(mg/ml) : 75
Solvent : DMSO (Dimethyl Sulfoxide)
Final Conc.(µg/ml) : 75
Description : Bacteriostatic. Dihydrofolate Reductase (dhfr) inactives trimethoprim and confers resistance.
Expiration Date: = 90 Days

Zeocin

Stock Conc.(mg/ml) : 100
Solvent : Water
Final Conc.(µg/ml) : 20-1000
Description : Zeocin binds and cleaves DNA, causing cell death. Resistance is conferred by ble, the bleomycin resistance gene found on Tn5.
Expiration Date: = 60 Days