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21331

LB Broth with Animal-Free Soytone. 1000mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$96.00

Fill Volume

1000 mL

COA
COA

21331

LB Broth with Animal-Free Soytone. 1000mL, Sterile.

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

LB Broth with Animal-Free Soytone is a nutrient broth medium formulated for cultivation and routine propagation of E. coli and other bacteria in liquid culture.

Animal-free soytone supplies peptides and amino acids, yeast extract contributes vitamins and growth factors, and sodium chloride maintains osmotic balance to support cellular growth.

Suitable for general cloning workflows, overnight cultures, and preparation of starter inocula; compatible with antibiotic supplementation for selection of plasmid-bearing E. coli when required. GMP manufactured.

  • This is a GMP-grade product that is manufactured in compliance with our Quality Management System in ISO 13485 certified facilities.
  • 1.69% Animal-Free Soytone
  • 0.50% Yeast Extract
  • 1.00% Sodium Chloride

  • Routine Bacterial Cultivation: Growth and maintenance of non-fastidious bacteria, especially E. coli, for general microbiology workflows.

  • Plasmid Propagation and DNA Prep: Overnight cultures for mini/midi/maxi preps to yield high-quality plasmid DNA from E. coli.

  • Antibiotic Selection: Selection and maintenance of recombinant strains carrying antibiotic resistance markers in E. coli.

  • Cloning and Subcloning Workflows: Starter and overnight cultures to support ligation, assembly, and screening of constructs.

  • Transformation Recovery: Recovery medium post heat-shock or electroporation to allow expression of resistance markers in E. coli.

  • Protein Expression Screening: Shake-flask cultures for small-scale expression testing in E. coli prior to scale-up.

  • Bacterial Library Maintenance: Culturing and passaging of cloning libraries and mutant libraries in E. coli.

  • Glycerol Stock Preparation: Growing robust overnight cultures for long-term cryostorage of E. coli strains.

  • Phage Work (Bacteriophage): Propagation and titering of bacteriophages that infect E. coli.

  • QC and Method Development: Standard growth control medium for method validation and instrument calibration using E. coli.

  • This is a GMP-grade product that is manufactured in compliance with our Quality Management System in ISO 13485 certified facilities.
  • pH (USP <791>)
  • Conductivity (USP <644>)
  • Performance
  • Sterility (USP <71>)
Storage Temperature
Room Temperature
Shelf Life
730 days
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