Lysogeny Broth (LB) is a nutritionally rich bacterial growth medium used for cultivating E. coli and other non-fastidious microorganisms. It’s commonly used in molecular biology workflows such as plasmid propagation, protein expression, and cloning.

Standard LB formulations contain 10 g of tryptone, 5 g of yeast extract, and 10 g of sodium chloride per liter. Variants such as LB Lennox Broth contain 5 g/L of sodium chloride, which can affect bacterial growth and recombinant protein yield.

Antibiotics are commonly added to LB media to maintain selective pressure for plasmid-containing or genetically modified bacterial strains. We carry antibiotic-free LB broth and formulations supplemented with ampicillin (50 µg/mL and 100 µg/mL), carbenicillin (50 µg/mL and 100 µg/mL), kanamycin (25 µg/mL and 50 µg/mL), Zeocin^Ⓡ (25 µg/mL), and chloramphenicol (25 µg/mL). We can also formulate LB broth with custom antibiotic combinations, adding up to 5 antibiotics to tailor your formulation to your needs. Learn more about all the antibiotics we offer.

LB media should typically be stored frozen or at room temperature, depending on the specific formulation. For more details, refer to the storage instructions provided with each individual product.

LB broth is a liquid medium that supports the growth of bacteria in suspension for applications like cloning or protein expression, while LB agar contains added agar to create a solid surface for isolating and maintaining individual bacterial colonies. Both share the same nutrient base, including tryptone, yeast extract, and sodium chloride, but differ in their physical state and use case. We sell liquid LB broth in tubes, bottles, and bags, as well as bottled LB agar (1.5% agar) and LB top agar (0.8% agar).

LB media is ideal for culturing, cloning, genetic engineering, and the expression of recombinant proteins in E. coli. It is a nutrient-rich formulation that promotes robust biomass accumulation, making it a dependable choice for workflows that require reliable transformation efficiency, plasmid maintenance, or high-yield protein production.

Yes, to save on preparation time and reduce contamination risk, we offer ready-to-use LB media in a wide variety of formulations and in formats ranging from tubes to bottles to bags. 

Absolutely. We are proud to offer industry-leading turnaround times for custom orders. You can easily tailor your media formulation, antibiotic combinations, pH levels, container formats, or fill volumes to meet the needs of your specific application.

Yes, each lot of our LB media has a Certificate of Analysis (COA) that confirms product quality, sterility, and performance specifications. COAs are available for download directly from our website or by request, ensuring full traceability and regulatory compliance for research and manufacturing environments.