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AAV Workflow

Solutions for every step

of your AAV workflow

 

 

Upstream. Downstream. And the downstream of the upstream process too.

Get all the AAV reagents you need from one trusted source, and learn how our 

AAV•Tek™ Solutions can save months in your process development at the polishing step.

 

Need solutions for plasmid production too? Explore our full suite of products for your plasmid workflow.

Using a different downstream processing method?
Cell Expansion and Transfection

We understand the importance of your upstream conditions and the critical nature of optimizing both the cell media and transfection reagent utilized. Our team of scientists, process engineers, manufacturing specialists, and quality control experts are here to help define and test custom reagents for your AAV needs.

 

Get in touch with our team to discuss the best solution for your specific application.

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See our findings on optimized formulations

Explore our research to learn more about how we can optimize our custom formulations for your cell expansion and transfection needs.

Cell Lysis and Endonuclease Treatment

Efficient cell lysis and endonuclease treatment are essential for maintaining high AAV yields and purity. We have proven cell lysis buffers in stock in multiple sizes to match your bioreactor needs, as well as Benzonase® Nuclease to reduce product related impurities such as residual host-cell DNA and plasmid.

 

Our Mammalian Cell Lysis Buffer is a polysorbate 20-based cell lysis reagent at a 10X concentration. The 100 mL volume is sufficient for 1 L of cell culture harvest, and the 500 mL volume is sufficient for 5 L of cell culture harvest.

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Filtration

Protect the integrity of your AAV particles while removing particulate contaminants by using this serotype-agnostic filtration buffer. Our AAV Filtration Buffer can be used both as an equilibration and chase buffer for your depth filtration membrane, and our AAV Stabilizer can reduce turbidity and pressure during filtration, preventing aggregation, and significantly increasing recovery of functional capsids.

Filtration buffer
Capture

Get every affinity buffer and reagent you need for the AAV capture step, including equilibration, wash, CIP, storage, neutralization, and conductivity adjustment solutions. Our AAV Affinity Buffer Kit buffers are also available individually, and you can use the AAV Stabilizer to protect the integrity of your capsids by promoting monodispersion during chromatography and reducing the potential for shear pressure induced loss of structural integrity.

 

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AAV polishing, simplified

Our proprietary AAV•Tek Solutions address current challenges in effective full capsid enrichment with our exclusive AEX Buffer Screening Kit, now available for AAV2, AAV6, and AAV8. Designed to help you quickly find the ideal buffer to optimize the separation of empty and full capsids while maintaining high yields, it can save months in your process development at the polishing step. 

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Storage

We have extensive experience manufacturing custom storage buffers. Our team of scientists, process engineers, manufacturing specialists, and quality control experts are here to help define custom storage reagents to meet your needs.

Fill out our custom order form or get in touch with our team to discuss the best solution for your specific application.

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Explore related products

All our products are fully customizable, from formulation to format to fill volume. Our experts can help find the ideal solution for your application and efficiently manufacture your custom product.

Discover more AAV•Tek Solutions

Get more reliable titer quantification

Improve your analytics and save time in your AAV sample preparation prior to PCR with our robust and streamlined AAV•Tek PCR Sample Prep Kit. Only from Teknova.

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Fully customizable

Need that in a 1 L bottle filled to 500 mL?
Or at a different pH for your DOE?


From formulation to format, quality control testing, documentation, and labeling, we can manufacture exactly what you need. Quickly tailor small volumes of buffers for your early-stage research with our Build•Tek™ Custom Configurator, or consult with our experts to find the best solution for your specific application.

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Our AAV experts are here to help every step of the way

Our scientists, process engineers, manufacturing specialists, and quality control experts are here to help. We can help you find the ideal solution for your specific application.

R&D team

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

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*Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany