Iodixanol Gradient Ultracentrifugation

We understand the importance of your upstream conditions and the critical nature of optimizing both the cell media and transfection reagent utilized. Our team of scientists, process engineers, manufacturing specialists, and quality control experts are here to help define and test custom reagents for your AAV needs.

Get in touch with our team to discuss the best solution for your specific application.

To purify your AAV, add the clarified cell lysate to a discontinuous iodixanol gradient. The 60% and 25% gradients contain phenol red to facilitate visualization of the different phases. Lower density contaminants, including empty AAV capsids, will gravitate toward the upper gradients (15-40%) during ultracentrifugation, while the 60% iodixanol gradient serves as a cushion for the full genome containing AAV capsids.

Tip: Keeping iodixanol gradient solutions refrigerated can make it easier to layer the solutions.

Our off-the-shelf iodixanol gradients include 15% iodixanol, 25% iodixanol with or without phenol red, 40% iodixanol, and 60% iodixanol with phenol red. 0.5% phenol red, PBS-MK, and PBS-MK with 1 M NaCl are also available to tailor your own gradient solutions.

For easy AAV diafiltration and concentration when using either a centrifugal filter or tangential flow filtration, we offer PBS and Poloxamer 188 concentrates so you can easily tailor your own solution.

TIP: multiple buffer exchanges may be required to completely remove the iodixanol.

We have extensive experience manufacturing custom storage buffers. Our team of scientists, process engineers, manufacturing specialists, and quality control experts are here to help define custom storage reagents to meet your needs.

Fill out our custom order form or get in touch with our team to discuss the best solution for your specific application.