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Super Broth Liquid Culture Media

Our super broth media is a nutrient-rich bacterial growth medium designed to support high-density cultures in molecular biology and cloning workflows. Super broth media are widely used for plasmid amplification, recombinant DNA applications, and bacterial culture when enhanced biomass and productivity are required.
Super Broth Liquid Culture Media
What is super broth media?

Super broth media, commonly referred to as SB broth, is a rich bacterial culture medium formulated with peptones (or alternatives), yeast extract, and buffering components that support robust bacterial growth. This nutrient-dense formulation promotes high cell densities and strong culture performance during extended incubation.


Super broth media is most commonly used to culture E. coli and other non-fastidious bacterial strains employed in cloning, plasmid propagation, and recombinant DNA workflows. Compared to standard LB broth, bacteria grown in super broth media often reach higher cell densities, supporting improved yields in downstream molecular biology and biotechnology applications.

Why choose Teknova’s super broth media?

Our super broth media, including formulations with animal-free soytone or without glucose, are supplied as a ready-to-use liquid medium, eliminating in-house media preparation and reducing variability. Each formulation is manufactured to precise specifications to ensure consistent nutrient availability and reproducible bacterial growth.

 

We manufacture our full catalog of super broth media using validated processes and rigorous quality control testing to ensure performance and lot-to-lot consistency. We also support custom manufacturing options, including modified super broth formulations, antibiotic supplementation (up to 5), alternative fill volumes, peptones, and bulk container formats to support specialized molecular biology and biotechnology workflows.

Explore related products

All our products are fully customizable, from formulation to format to fill volume. Our experts can help find the ideal solution for your application and efficiently manufacture your custom product.

Frequently asked questions

You can find answers here for our most commonly asked questions. If you don't find the information you are looking for, get in touch with our team. We're here to help.

Super broth media is used for high-density bacterial growth in cloning, plasmid propagation, and recombinant DNA workflows, particularly with E. coli strains.

Super broth media supports the growth of non-fastidious bacteria, most commonly E. coli strains used in molecular biology and genetic engineering applications.

Super broth media is richer than LB broth and contains higher nutrient concentrations, supporting higher cell density and improved bacterial growth.

Super broth media should be stored according to product specifications, typically at room temperature, to maintain performance and shelf life. The storage instructions and expiration date for your specific product can be found on your product label.

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

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1.800.209.4488

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