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Peptones

Everything you need to know about peptones

Peptones (protein hydrolysates) are widely used as a protein source in culture media. These complex mixtures of polypeptides and amino acids are rich in water-soluble minerals and micronutrients derived from the original protein source.

 

They provide critical nutritional support for cell growth and proliferation, microbial fermentation, and production of recombinant proteins.

Peptones

 

 

 

 

Types of peptones

There are five commonly used peptones that originate from plant or animal sources. They differ in nitrogen and amino acid content.

Meat Peptone is an enzymatic digest of animal bovine tissue (e.g., muscle, bone, skin, and pancreas). It is used for the cultivation of fastidious and non-fastidious microorganisms.

Soytone (soy peptone) is a pancreatic digest of defatted soybean meal. It is a good sources of vitamins and carbohydrates, and is used in the cultivation of a wide variety of microorganisms, including bacteria and fungi.

Animal-free soytone is a papain (from papaya) digest of defatted soybean meal.

Tryptone is a pancreatic digest of casein (a dairy protein) containing all amino acids and larger peptide fractions. This nutrient source is commonly used in media for production of antibiotics, toxins, enzymes, and other biological products through fermentation.

Casamino acid and casein are often used interchangeably, as casamino acid is the hydrolysate derived from casein breakdown. Casein is a protein found in milk and its acid hydrolysis results in an amino acid mixture. When used in cell culture media, it provides a rich source of essential nutrients to foster the growth of diverse microorganisms and support protein synthesis.

Formulation details

 

See the chart below for an estimate of the nitrogen and amino acid profiles for different types of peptones.

Explore our peptone products

 

All our products are fully customizable, from formulation to format to fill volume. Our experts can help find the ideal solution for your application and efficiently manufacture your custom product.

 

 

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

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