Galactose-Inducible Expression in Yeast: Supports induction of GAL1/GAL10-driven constructs in Saccharomyces cerevisiae using 2.00% D-(+)-Galactose.

Yeast Cultivation and Maintenance: Provides a defined base (0.67% Yeast Nitrogen Base) with amino acids (0.50% Casamino Acids) for routine growth of laboratory yeast strains.

Protein Production in Yeast: Enables galactose-induced expression for recombinant protein and secreted protein workflows.

Metabolic and Regulatory Studies: Used to study galactose utilization pathways and relief of glucose repression in yeast.

Yeast Two-Hybrid and Reporter Assays: Facilitates galactose-dependent activation of GAL-promoter reporters in assay workflows.

Pre-Cultures and Carbon Source Shift Experiments: Employed for preculturing and switching from glucose to galactose to initiate induction.

Transformation Recovery and Plasmid Maintenance: Serves as a buffered recovery medium post-transformation prior to selection.

pH-Stable Yeast Growth: 0.54% Disodium Phosphate and 0.86% Monosodium Phosphate provide buffering for consistent culture pH.