2xYT agar plates are a nutrient-rich, general-purpose, solid bacterial culture medium composed of yeast extract, tryptone, sodium chloride, and agar. This enriched composition provides elevated levels of amino acids, peptides, vitamins, and growth factors compared to LB agar, thereby supporting increased bacterial biomass and faster growth.

2xYT agar plates are commonly used for cultivating E. coli and other non-fastidious bacteria in applications such as cloning, plasmid amplification, strain maintenance, recombinant protein expression, phage propagation, and phage display. 2xYT agar may be used as a non-selective growth medium or supplemented with antibiotics for selection during genetic manipulation and molecular cloning experiments.

Our 2xYT agar plates are supplied as ready-to-use pre-poured plates, eliminating in-house preparation time and reducing batch-to-batch variability in molecular biology and microbiology workflows. We provide a wide variety of 2xYT agar plates in multiple formulations, including additives such as glucose to support catabolite repression of toxic recombinant proteins, and antibiotics, including carbenicillin, kanamycin, ampicillin, tetracycline, chloramphenicol, and various combinations.

Our extensive portfolio of 2xYT agar plates is manufactured under validated, rigorous quality control processes to ensure sterility, performance, and lot-to-lot consistency. We also provide custom manufacturing services, including modified formulations, antibiotic combinations (up to 5), peptone sources, and alternative plate formats to support specialized 2xYT agar requirements across academic and industrial laboratories and bioprocessing facilities.