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AAV●Tek AAV Stabilizer

Protect the integrity of your AAV capsid

Get up to a 50% increase in yield with our novel AAV●Tek™ AAV Stabilizer. This multi-purpose buffer safeguards your product across your AAV purification workflow, reducing turbidity and pressure during filtration, preventing aggregation, and significantly increasing recovery of functional capsids.

AEX buffer screening kit with box

 

NOW AVAILABLE

 

AAV●Tek AAV Stabilizer

 

 

 The AAV●Tek AAV Stabilizer offers a multi-purpose solution that can help you achieve substantial recovery improvements and safeguard your product. It minimizes aggregation effects on surfaces, reduces turbidity and pressure during filtration, and promotes monodispersion during chromatography. It also reduces the potential for shear pressure induced loss of structural integrity (e.g., unfolding, rupturing, precipitation, etc.), and can improve recovery by reducing unwanted binding interactions of your product with surfaces.

 

This 100x concentrated solution is available in 500 mL and 100 mL formats that can be directly spiked into your feed stock, buffers, and solutions to enhance capsid integrity.

 

 

 

 

Ensure your

AAV capsid integrity

 

Save time and resources with this solution-based safety factor that can be easily used as a spiked additive to downstream bioprocess buffers and solutions.

Prevent aggregation

 

Prevent and reverse aggregation of your AAV particles. This solution can facilitate AAV monodispersion by reducing non-specific binding to surfaces and minimizing interfacial forces.

Reduce membrane pressure 10-40%

 

Reduce the risk of losing valuable material through membrane fouling and capsid degradation. By providing shielding during filtration this solution reduces pressure and turbidity.

Increase yield by up to 50%

Combine the benefits of aggregation prevention and membrane pressure reduction during filtration with the reduction of AAV particle adsorption to surfaces through shielding.

See the data

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The table below shows the cumulative beneficial effects of incorporating the AAV Stabilizer into your AAV purification workflow. The addition of this formulation can result in an average 16% reduction in turbidity, 15% reduction in membrane pressure, 23% reduction in aggregation, 40% increase in recovery, and a 20% increase in infectivity.     ​

Turbidity reduction was measured by comparing the turbidity of the filtrates from different filter trains that were challenged with different serotype AAVs treated with and without the AAV Stabilizer during the filtration of harvest lysate. Serotypes AAV6 and AAV9 showed the highest turbidity reductions during clarification.

 

Pressure reduction was measured by comparing the average pressure of filters being challenged with different serotype AAVs treated with and without the AAV Stabilizer during the filtration of harvest lysate. Serotypes AAV2 and AAV8 showed the highest average pressure reductions during clarification.

The components in the AAV Stabilizer facilitate AAV monodispersion by preventing non-specific binding to surfaces and minimizing interfacial forces. It can both prevent and reverse the aggregation of your AAV particles.  

Figure 3: Dynamic Light Scattering (DLS) overlay of AAV2 containing affinity eluate (-AAV Stabilizer; orange) and AAV2 containing affinity eluate (+AAV Stabilizer; blue). Affinity eluate produced without the AAV Stabilizer showed 38% aggregation. Affinity eluate produced with the AAV Stabilizer showed no aggregation.

 

 

Figure 4: Aggregation reduction was measured by comparing the aggregation levels by DLS of affinity to filtrates of the eluates from different serotype AAVs treated with and without the AAV Stabilizer. Serotypes AAV2 and AAV8 showed the highest reduction of aggregation.

Increased recovery was observed during and after the affinity chromatography step in the presence of the AABV Stabilizer. This is shown below using DLS data across multiple tested serotypes.

Figure 5:

Left: Dynamic Light Scattering (DLS) overlay of AAV2 containing affinity eluate that was not treated with the AAV Stabilizer, showing a significant loss occurring during filtration (orange). 

 

Right: Dynamic Light Scattering (DLS) overlay of AAV2 containing affinity eluate that was treated with the AAV Stabilizer, showing no loss of AAV particles during filtration.  

 

 

 

Figure 6: Affinity recovery improvement was measured by comparing the recovery of different serotype AAVs treated with and without the AAV Stabilizer during affinity chromatography. Serotypes AAV6 and AAV9 showed the highest average recovery increases.

Infectivity improvement was measured by comparing the infectivity recovery of transduction units from lysate to affinity eluate filtrate from processes using different serotype AAVs treated with and without the AAV Stabilizer. Serotypes AAV8 and AAV9 showed the highest infectivity improvements.

 

How it works

The special properties of poloxamer and sucrose help stabilize AAV particles during filtration.

 

Aggregation of proteins at the membrane surface causes excessive turbulent flow and shear forces. These forces act tangentially to the membrane and can disrupt the structure of AAV.

 

Poloxamer shields AAV from non-specifically binding to tubing material and filter membranes, allowing the particles to flow as monodispersed particles.

 

Sucrose acts as a highly viscous amorphous environment on the surface of AAV to provide conformational stability and reduce capsid rupture.

 

Too high a concentration of these excipients could adversely affect binding of AAV to resins or the enhanced viscosity could affect filtration. We have determined that the addition of 0.01% Poloxamer and 0.1% Sucrose works well for filtration and chromatography.

 

Use the AAV Stabilizer throughout

your AAV workflow

 

Explore related products

All our products are fully customizable, from formulation to format to fill volume. Our experts can help find the ideal solution for your application and efficiently manufacture your custom product.

Frequently asked questions

You can find answers here for our most commonly asked questions. If you don't find the information you are looking for, get in touch with our team. We're here to help.

The AAV●Tek AAV Stabilizer has a proprietary formulation that contains poloxamer and sucrose. Please get in touch if you'd like more information or have any questions.

Spiking the AAV Stabilizer into the lysate load, filtration buffers, affinity chromatography buffers, and affinity product filtration buffers is a great way to protect the integrity of your AAV capsid.

We have shown that the AAV Stabilizer increases recovery and concomitantly infectivity of AAV capsids.​

The AAV Stabilizer is provided as a 100x concentrate. For example, 10 mL of AAV Stabilizer should be added to a 1000 mL volume.

We have not encountered any matrix effects of AAV Stabilizer ingredients on standard AAV assays. ​

The people behind our novel products

Meet the AAV team

Our AAV●Tek Solutions are designed by our dedicated Research and Development team as part of our initiative to invest in the discovery of novel products designed to give you a competitive advantage in your work.

 

With custom manufacturing capabilities that fit seamlessly into your cell and gene therapy workflow and our in-house scientific expertise, we can be a true partner to you, helping you overcome bioprocessing challenges and accelerate your breakthroughs.

Group shot of the research and development team

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

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