Cultivation of Fastidious Organisms: Supports primary isolation and robust growth of fastidious bacteria from clinical and environmental specimens using a TSA base with 5% defibrinated sheep’s blood.

Hemolysis Differentiation: Enables visualization of alpha, beta, and gamma hemolysis patterns for presumptive identification of streptococci and other hemolytic species.

Throat and Respiratory Pathogen Isolation: Recovery and presumptive identification of beta-hemolytic streptococci such as Streptococcus pyogenes and alpha-hemolytic species like Streptococcus pneumoniae.

Staphylococcal Isolation: Isolation and assessment of hemolytic activity of Staphylococcus aureus from wound, blood, and respiratory specimens.

Listeria Screening: Observation of narrow-zone beta-hemolysis to aid in detection of Listeria monocytogenes from food and clinical samples.

Satellitism Test: Detection of growth enhancement (satellitism) of Haemophilus influenzae around Staphylococcus aureus streaks for presumptive identification.

Colony Morphology and Pigment Assessment: Evaluation of colony size, color, hemolysis, and odor for preliminary microbial identification.

Antimicrobial Susceptibility Screening: Qualitative disk diffusion screening for fastidious organisms when method and supplement guidelines permit.

Enumeration and Bioburden Testing: CFU counts and microbial load assessment in clinical, food, and environmental monitoring workflows.

Subculture and Maintenance: Routine subculture and short-term maintenance of clinical isolates requiring enriched, non-selective media.