Yeast Cultivation and Plating: Supports robust growth of Saccharomycetales using glucose, YNB with ammonium sulfate, and casamino acids.

Transformant Recovery: 1 M sorbitol provides osmotic stabilization for recovery after lithium acetate/PEG or electroporation-based yeast transformations.

Spheroplast Handling: Maintains viability of yeast spheroplasts during plating and recovery.

Colony Isolation and Titering: Enables single-colony isolation and CFU determination for yeast libraries and clones.

Yeast Display Workflows: Used for growth under glucose-mediated GAL promoter repression prior to induction on galactose media.

Selective Screening with TRP1: Casamino acids (tryptophan-poor) permit TRP1-based selection while complementing most other auxotrophies.

Cell Wall Mutant Phenotyping: Osmotic support facilitates screening of cell wall–defective or osmo-fragile yeast strains.

Replica Plating and Strain Purification: Suitable for transferring and purifying yeast strains across plates.

Pichia pastoris Workflows: Supports transformation recovery and clone screening in methylotrophic yeast.

Short-Term Stock Maintenance: Preserves yeast cultures on plates for routine lab use.