Routine Bacterial Cultivation: General-purpose liquid medium for growing non-fastidious bacteria such as Escherichia coli and related enteric species.

Plasmid Propagation and Cloning: Supports high-density growth for plasmid maintenance and amplification in E. coli-based cloning workflows.

Antibiotic Selection in Liquid Culture: Used with selective antibiotics to maintain plasmids or selectable markers in E. coli cultures.

Starter and Overnight Cultures: Preparation of inocula for downstream applications including cloning, sequencing, and protein expression.

Transformation Recovery/Outgrowth: Short post-transformation recovery of E. coli prior to plating or selection.

Protein Expression Pre-cultures: Growth of seed cultures of recombinant E. coli for subsequent induction in production media.

Glycerol Stock Preparation: Culturing cells prior to cryopreservation of E. coli strains and plasmid libraries.

Educational and QC Applications: Standard medium for teaching labs and routine strain maintenance/verification in microbiology.