Antimicrobial Susceptibility Testing (AST): Standard medium for Kirby–Bauer disk diffusion; cation adjustment supports accurate results for aminoglycosides and tetracyclines against organisms like Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.

Broth Microdilution MIC Testing: Used to determine minimum inhibitory concentrations of antibiotics for nonfastidious bacteria (e.g., E. coli, Enterococcus faecalis, P. aeruginosa).

Broth Macrodilution MIC/MBC: Supports tube dilution and bactericidal (MBC) assays for comparative potency and kill-curve assessments.

Quality Control of AST: Media and antibiotic performance verification with QC strains such as E. coli ATCC 25922, S. aureus ATCC 29213/25923, and P. aeruginosa ATCC 27853.

Growth of Nonfastidious Bacteria: General-purpose cultivation and maintenance in log phase for organisms like Staphylococcus aureus, Escherichia coli, and Enterococcus faecalis.

Colistin/Polymyxin MIC Testing: Cation-adjusted formulation supports reliable susceptibility testing for polymyxins against Gram-negative bacteria (e.g., E. coli, P. aeruginosa).

Time–Kill Kinetics Studies: Evaluates bactericidal activity of antimicrobials over time in standardized broth conditions.

Antibiotic Potency and Stability Assays: Serves as a defined test matrix to assess antimicrobial activity and lot-to-lot consistency.

Inoculum Preparation for AST: Supports preparation of standardized bacterial suspensions for downstream susceptibility testing.

Resistance Surveillance and Method Validation: Used in clinical and research labs for benchmarking new methods and monitoring resistance trends in organisms like E. coli, S. aureus, and P. aeruginosa.