Defined Bacterial Cultivation: Growth of Escherichia coli and other enteric bacteria in a minimal, defined medium with glycerol as the carbon source.

Recombinant Protein Expression: Supports expression in Escherichia coli while minimizing catabolite repression due to glycerol instead of glucose.

Plasmid Maintenance and Selection: Base medium for antibiotic selection and stable plasmid maintenance under defined conditions.

Auxotroph Support: Growth of thiamine-requiring strains and amino acid-demanding mutants via 0.5 µg/mL thiamine and 2% casamino acids.

Metabolic and Physiology Studies: Evaluation of carbon utilization, respiration, and stress responses in minimal salts with defined supplements.

Bacteriophage Assays: Adsorption and propagation assays (e.g., lambda/P1) facilitated by 0.2 mM MgSO4 and 0.1 mM CaCl2.

Fermentation and Scale-Up: Starter cultures for fed-batch processes using glycerol-based feeds for controlled growth.

Growth of Sensitive Constructs: Culturing strains expressing toxic or burdensome proteins where slower, glycerol-based growth improves stability.