Bacterial Culture Supplementation: Enriches minimal media (e.g., M9) with readily assimilable amino acids/nitrogen to support robust growth of Escherichia coli and other bacteria.

Auxotroph and Nutritional Studies: Essentially tryptophan-free; used for tryptophan auxotroph selection and defined supplementation experiments.

Yeast Media Preparation (CAA/SD-CAA): Combined with yeast nitrogen base to formulate casamino-acid media for growth and selection of Saccharomyces cerevisiae.

Phage Work and Plaque Assays: Supplements bacterial lawns or top agar to enhance plaque formation and yields with hosts such as E. coli.

High-Density Starter and Expression Cultures: Shortens lag and supports vigorous growth in defined/minimal media for recombinant protein expression in E. coli.

Cultivation of Fastidious Microorganisms: Added to media to support organisms with complex amino acid requirements.