Yeast Cultivation and Propagation: Defined medium for robust growth of Saccharomyces cerevisiae and related yeasts with controlled carbon and nitrogen sources.

Yeast Surface Display Workflows: Standard growth medium for library expansion and selection prior to galactose-based induction.

Auxotrophic Selection Assays: Supports selection schemes leveraging casamino acids (low in tryptophan) with YNB (w/o amino acids, w/ ammonium sulfate).

Glucose-Repression and Metabolic Studies: 2% glucose enables controlled repression of GAL promoters for regulatory and metabolic experiments.

Protein Expression Screening in Yeast: Used for pre-induction growth and plasmid maintenance in cloning and expression workflows.

Quantitative Growth and Fitness Assays: Chemically defined formulation enables reproducible OD-based growth curves and competition assays.

Phosphate-Buffered, pH-Stable Culturing: Disodium and monosodium phosphate provide buffering for consistent pH during cultivation and assays.

Strain Banking and Starter Cultures: Suitable for preparing glycerol stocks and inocula for downstream yeast experiments.