Antimicrobial Susceptibility Testing (Kirby–Bauer Disk Diffusion): Standardized, cation-adjusted medium for reproducible inhibition zones with organisms like Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis.

Gradient Diffusion MIC (Etest): Determines minimum inhibitory concentrations on uniform lawns of nonfastidious bacteria.

Antibiotic Potency and QC Testing: Used with reference strains (e.g., Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212).

Cultivation and Isolation of Nonfastidious Bacteria: Supports robust growth for routine culture and subculture of organisms such as Escherichia coli and Staphylococcus aureus.

Colony Enumeration and Purity Checks: Used for CFU counts, streak isolation, and verification of culture purity from clinical, environmental, or food samples.

Antimicrobial Peptide/Bacteriocin Assays (Spot-on-Lawn): Evaluates activity of antibiotics and peptides against standardized bacterial lawns.

Combination/Synergy Testing: Assesses interactions of antimicrobial agents using disk proximity or strip overlays on consistent growth medium.

Testing Fastidious Organisms with Supplements: Basis for HTM-like applications when supplemented (e.g., susceptibility testing of Haemophilus influenzae and Neisseria gonorrhoeae).