Defined Growth of Escherichia coli: Routine cultivation in a minimal, glucose-defined medium supplemented with casamino acids and thiamine.

Auxotroph Selection and Screening: Identification of amino acid auxotrophies (commonly leveraging casamino acids that are low in tryptophan) in enteric bacteria.

Recombinant Protein Production: Controlled expression in E. coli under minimal conditions to reduce background metabolites and improve reproducibility.

Metabolomics and Flux Analysis: Experiments requiring defined nutrients (1X M9 salts, glucose, Mg2+, Ca2+, thiamine) for consistent metabolic baselines.

Plasmid Maintenance with Antibiotic Selection: Stable growth of recombinant strains in a chemically defined medium for consistent selection pressure.

Carbon Source Utilization Studies: Assessment of glucose-dependent growth phenotypes and catabolite repression effects in E. coli.

Cultivation of Other Enteric Bacteria: Growth of organisms such as Salmonella and Shigella in a defined broth with essential ions (Mg2+, Ca2+).