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21337

50% (v/v) Glycerol Solution, 250mL Sterile.

Availability

Ships in 6 - 14 Days

Container Size

250mL

Item Count

1 Bottle(s)

Price per Item

$95.00

Fill Volume

250 mL

21337

50% (v/v) Glycerol Solution, 250mL Sterile.

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

50% (v/v) Glycerol Solution is an aqueous glycerol preparation for laboratory use in cryopreservation workflows, buffer formulation, and reagent storage. It is suitable as a cryoprotective additive for microbial and cell culture applications and for stabilizing enzymes and proteins in storage buffers.

The 50% concentration allows straightforward dilution to common working ranges (e.g., 10–25% v/v) for preparing frozen stocks of E. coli and other microorganisms, and for adjusting osmotic strength in biochemical assays. GMP manufactured.

  • This is a GMP-grade product that is manufactured in compliance with our Quality Management System in ISO 13485 certified facilities.
  • 50% (v/v) Glycerol
  • Enzyme and Protein Stabilization: Added to storage buffers (often 50% v/v) to prevent freezing at −20°C and maintain enzyme/protein activity.

  • Cryopreservation of Bacterial Stocks: Used at 10–25% v/v for long-term −80°C storage of strains such as Escherichia coli and Bacillus subtilis.

  • Cryopreservation of Mammalian and Yeast Cells: Component of cell-freezing media to reduce ice crystal formation and improve post-thaw viability.

  • Preparation of Electrocompetent Cells: Utilized in cold, isotonic washes (typically 10% v/v) to reduce conductivity prior to electroporation.

  • PCR/qPCR Additive: Enhances amplification of difficult or GC-rich templates and stabilizes polymerases in reaction mixes.

  • DNA/RNA Storage: Included in nucleic acid buffers to improve stability during freezing and thawing.

  • Gel Loading Buffer Component: Increases sample density for reliable well loading in agarose or polyacrylamide gels.

  • Glycerol Gradients/Cushions: Used to create density gradients or cushions for ultracentrifugation of macromolecules and ribonucleoproteins.

  • Protein Purification Workflows: Added to chromatography buffers to minimize aggregation and maintain protein solubility.

  • Carbon Source in Microbial Culture: Serves as a defined carbon source for organisms such as Escherichia coli, Pseudomonas aeruginosa, and Mycobacterium smegmatis.

  • GMP/Regulated Workflows: Suitable for use in GMP settings; manufactured under an ISO 13485-certified QMS and supplied sterile.

  • This is a GMP-grade product that is manufactured in compliance with our Quality Management System in ISO 13485 certified facilities.
  • Osmolality (USP <785>)
  • Density (USP <841>)
  • Sterility (USP <71>)
Storage Temperature
Room Temperature
Shelf Life
1095 days
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