pH Adjustment and Titration: Standard base for adjusting pH of buffers, media, and reagents, and for acid-base titrations.

Chromatography Column Cleaning/Sanitization: Used to clean and sanitize anion-exchange and affinity resins, tubing, and systems between runs.

CIP of Bioprocess Equipment: Applied in cleaning-in-place cycles for tanks, bioreactors, and transfer lines to remove organic residues.

Endotoxin Reduction on Surfaces: Employed to reduce endotoxin burden on labware and system components with appropriate contact time.

Alkaline Denaturation of Nucleic Acids: Utilized for DNA/RNA denaturation in alkaline blotting and preparation of alkaline agarose gels.

Plasmid Alkaline Lysis: Component of lysis solutions for plasmid extraction from Escherichia coli cell pellets.

Media and Feed pH Control in Fermentation: Adjusts culture pH during growth of organisms such as Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa.

Neutralization/Quenching of Acidic Fractions: Neutralizes acidic eluates (e.g., TFA-containing HPLC fractions) and acid-quenched reactions.

Resin and System Storage: Sets alkaline storage conditions to inhibit microbial growth in columns and LC systems.

Base Hydrolysis/Saponification: Performs alkaline hydrolysis steps in sample preparation and analytical workflows.