Polymerase Chain Reaction (PCR): Used in PCR reactions to maintain optimal pH conditions for enzyme activity.

Gel Electrophoresis: Serves as a component in Tris-based running and sample buffers, providing consistent pH for nucleic acid and protein separation.

Protein Purification: Utilized in anion exchange chromatography to maintain a stable alkaline pH environment during protein binding and elution.

Enzyme Assays: Provides a consistent alkaline pH for enzymatic reactions (e.g., alkaline phosphatase), ensuring accurate kinetic measurements.

Nucleic Acid Purification: Used for preparation of Tris-based elution and resuspension buffers to stabilize DNA and RNA.

Western Blotting: Used to prepare Tris-based transfer and wash buffers, maintaining pH for efficient protein transfer and antibody binding.