Chromatography Column Storage: Used to store HPLC/FPLC columns in 20% (v/v) Ethanol to inhibit microbial growth during downtime.

FPLC/HPLC System Preservation: Circulated as a hold solution in pumps, valves, and lines to deter biofilm formation between runs.

Bioprocess Fluid-Path Wet Hold: Maintains sterility of single-use tubing, manifolds, and filters with 20% (v/v) Ethanol between campaigns.

Process Line Flushes: Applied as a final aqueous-organic flush to reduce bioburden and displace water prior to storage or sterilization.

Buffer/Media Preservation: Added at 20% (v/v) to aqueous solutions for short-term storage to inhibit growth of microbes such as Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.

Sample Hold/Transport: Mixed at 20% (v/v) with aqueous samples to limit microbial proliferation during short-term handling.

Equipment Soak: Used as a soak solution for small parts and fittings to minimize microbial contamination risk between uses.