Polymerase Chain Reaction (PCR): Used in PCR/qPCR mixes to maintain alkaline pH for optimal polymerase performance.

Gel Electrophoresis: Preparation of Tris-based running buffers (e.g., Tris-Glycine, Tris-Borate) for SDS-PAGE and native PAGE.

Protein Purification: Buffering for anion exchange chromatography and protein dialysis/storage at alkaline pH.

Enzyme Assays: Provides stable pH 9 conditions for alkaline-active enzymes (e.g., alkaline phosphatase assays).

Nucleic Acid Purification: pH control for DNA elution and resuspension buffers in plasmid and genomic DNA workflows.

Western Blotting: Preparation of Tris-Glycine transfer buffer and Tris-buffered saline (TBS) from concentrated stock.

General Buffer Preparation: Convenient stock for making Tris-based buffers at pH ~9 for biochemical and GMP workflows.