Column Equilibration: Prepares anion exchange (AEX) columns for AAV6 purification by setting target pH and conductivity prior to loading.

AAV6 Binding Optimization: Establishes conditions that promote AAV6 capsid binding to AEX media for capture or polishing steps.

Feedstream Conditioning: Dilutes or adjusts process intermediates (e.g., TFF retentate) to the appropriate ionic strength before AEX loading.

Column Washing: Performs low‑stringency washes to clear host‑cell impurities and process contaminants while maintaining AAV6 on the column.

Process Consistency and Scale‑Up: Supports reproducible AEX workflows from development to GMP manufacturing by standardizing equilibration conditions.

Method Qualification and QC: Used as the equilibration buffer in validated AEX methods for system suitability and routine quality control.