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3S8000

Studier ZYP-5052 Medium Kit. 1L Kit

Availability

Ships in 1 - 2 Days

Container Size

1L

Item Count

1 Kit(s)

Price per Item

$73.00

COA
COA

3S8000

Studier ZYP-5052 Medium Kit. 1L Kit

Quantity

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What’s in the Kit
Description
Formulation
Common Applications
Storage

This kit includes the following products:

 

1 x  3S8600: Studier ZYP-5052 Salts Solution, 120 mL
1 x  3S8200: Studier ZYP-5052 Medium, 880 mL

Studier ZYP-5052 Medium Kit, 1 L Kit is a defined autoinduction medium kit assembled to prepare 1 liter of ZYP-5052 medium.

This medium is used for cultivation of E. coli and lactose-based autoinduction of recombinant protein expression from lac/T7 promoter systems in plasmid-bearing strains.

  • Studier ZYP-5052 Medium Kit
  • To complete media aseptically add 120mL of 3S8600 to 3S8200

  • Autoinduction of recombinant proteins in Escherichia coli (E. coli): Lactose-based, IPTG-free induction for T7-expression strains (e.g., BL21(DE3)).

  • Overnight expression in shake flasks: Robust growth and gradual induction to improve soluble protein yields.

  • High-throughput expression screening: Consistent autoinduction in 24/96 deep-well plate formats.

  • Toxic or membrane protein expression: Slow, steady induction to reduce metabolic burden and improve viability.

  • Starter and scale-up cultures: Seamless transition from small-scale tests to liter-scale production.

  • Expression in common T7 strains: Optimized for Escherichia coli BL21(DE3), T7 Express, and similar hosts.

  • Media preparation check: Use Studier ZYP-5052 Medium Kit; to complete media aseptically add 120mL of 3S8600 to 3S8200.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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