AEX Column Equilibration for AAV2: Establishes target pH and conductivity on anion-exchange media prior to AAV2 loading.

Sample Conditioning for AAV2 Loading: Used to dilute or buffer-exchange AAV2 material to match AEX binding conditions.

Chromatography System Priming: Primes pumps, lines, and detectors to stabilize baseline pH and conductivity before runs.

Column Washing Under Binding Conditions: Maintains binding environment while removing unbound or weakly bound impurities.

Gradient Start/Run Buffer: Serves as the starting buffer for salt gradients during AEX separations.

In-Process Hold Between Steps: Maintains column stability and reproducibility during short holds as defined by SOPs.

Method Development and Tech Transfer: Provides a standardized equilibration matrix for screening and scale-up of AAV2 AEX methods.