Column Equilibration (AEX): Prepares anion exchange columns for loading AAV2 by setting initial pH and conductivity conditions.

Column Conditioning/Stabilization: Establishes a stable baseline and consistent binding environment between runs.

Sample Preparation/Buffer Exchange: Dilutes or adjusts feed streams into AEX starting conditions prior to loading.

Impurity Wash Steps: Used in wash phases to remove host cell proteins, DNA, and process-related impurities while retaining AAV2 on the column.

Process Development: Supports method optimization and robustness studies for AEX-based AAV2 purification.

Scale-Up/Manufacturing: Enables consistent column equilibration across pilot and GMP production runs.

Re-Equilibration: Restores initial conditions between gradient steps or after column regeneration.

System Suitability/Validation: Used during system checks and validation runs to verify AEX performance for AAV2 workflows.