Cryopreservation of Microbial Stocks: Prepares long-term glycerol stocks of bacteria (e.g., Escherichia coli, Bacillus subtilis) and yeast by diluting 50% (v/v) Glycerol to 10–25% final.

Competent Cell Preparation and Electroporation: Uses 50% (v/v) Glycerol to make 10% glycerol washes/resuspensions for low-conductivity handling of E. coli and other bacteria.

Enzyme and Protein Stabilization: Adds 10–50% glycerol (from 50% (v/v) Glycerol stock) to storage buffers to prevent freezing and stabilize enzymes/proteins at −20 °C.

Gel Electrophoresis Sample Loading: Increases sample density by adding glycerol (from 50% (v/v) Glycerol) to DNA/RNA/protein loading buffers for reliable well loading.

Protein Purification and Storage Buffers: Incorporates glycerol (via 50% (v/v) Glycerol stock) to reduce aggregation and enhance protein stability during purification and storage.

PCR/qPCR Additive: Supplements reactions with small percentages of glycerol (prepared from 50% (v/v) Glycerol) to aid amplification of difficult templates and stabilize polymerases.

Cryoprotection in Structural Biology: Serves as a cryoprotectant (typically 10–30% from 50% (v/v) Glycerol) for protein crystals and samples prior to flash-freezing.

Microbial Carbon Source: Provides a defined carbon source in culture media (e.g., glycerol minimal media for Escherichia coli) by dosing from 50% (v/v) Glycerol to 0.2–2% final.

Phage and Viral Stock Stabilization: Protects bacteriophage and viral preparations during freezing by adding glycerol derived from 50% (v/v) Glycerol.