Plasmid Selection in E. coli: Selects for colonies harboring kanamycin-resistance plasmids on LB agar (1.0% tryptone, 0.5% yeast extract, 1.0% NaCl, 1.5% agar) with 30 µg/mL kanamycin.

Bacterial Transformation Screening: Enables colony selection after heat-shock or electroporation with kanamycin-resistant constructs.

Colony Isolation and Maintenance: Streaking and passaging of kanamycin-resistant E. coli strains to maintain plasmid selection.

CFU Enumeration under Selection: Quantifies transformation or conjugation efficiency by counting kan-resistant colonies.

Conjugation/Transposition Selection: Recovers recipients that acquired kan-marked elements (e.g., Tn5-based) on selective LB plates.

Colony PCR and Sequencing Prep: Picks kan-resistant colonies for insert verification and downstream sequencing.

Pre-culture Setup for Protein Expression: Isolates kan-resistant E. coli clones carrying expression plasmids prior to induction in liquid culture.

CRISPR/Edit Selection: Selects edits or integrations using kanamycin resistance cassettes in E. coli.

Replica Plating for Plasmid Stability: Screens for maintenance of kan-resistant plasmids across passages.

Strain Verification and Archiving: Confirms kan phenotype and prepares selective streak plates for short-term storage.