PCR Optimization and Error-Prone PCR: Added as a Mn2+ cofactor to modulate DNA polymerase fidelity, enhance amplification of difficult templates, and increase mutation rates in error-prone PCR.

Reverse Transcription: Used to enable or improve RT performance on structured RNA templates by substituting or supplementing Mg2+ with Mn2+.

Nuclease and Restriction Enzyme Assays: Employed to activate or alter specificity of nucleases (e.g., DNase I) and certain restriction enzymes that accept Mn2+.

Glycosyltransferase Reactions: Provides essential Mn2+ cofactor for many glycosyltransferases in glycan synthesis and protein glycosylation assays.

Metalloenzyme Activity Assays: Supplies Mn2+ for manganese-dependent enzymes (e.g., MnSOD, arginase) to assess kinetics and cofactor requirements.

Bacterial Competent Cell Preparation: Included in transformation buffers to improve DNA uptake in Escherichia coli and Bacillus subtilis.

Microbial Culture Media Supplementation: Added as a trace metal source to support growth and metabolism in bacteria (e.g., E. coli, Bacillus spp.) and yeast fermentations.

Protein Crystallography and Structural Studies: Used to replace Mg2+ or stabilize metal-binding sites, aiding crystal formation and mechanistic trapping.

CRISPR and Nucleic Acid Editing Systems: Mn2+ supplementation can modulate activity and specificity of certain CRISPR-associated nucleases.

Aptamer and Ribozyme Assays: Provides Mn2+ to support folding and catalytic activity in RNA/DNA aptamers and ribozymes.