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T5821

Trypticase Soy Broth (TSB), 450 ml

Availability

Ships in 6 - 14 Days

Container Size

650mL

Item Count

1 Bottle(s)

Price per Item

$35.00

Fill Volume

450 mL

T5821

Trypticase Soy Broth (TSB), 450 ml

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

Trypticase Soy Broth (TSB) is a general-purpose, non-selective liquid medium used for the cultivation of a broad range of aerobic and facultative anaerobic bacteria from clinical, environmental, and industrial samples. The formulation provides peptides and amino acids from enzymatic digests of casein and soybean, a carbohydrate source, sodium chloride for osmotic balance, and phosphate for buffering. The medium is commonly employed for routine cultivation, inoculum preparation, sterility testing, and microbial enumeration.

TSB supports growth of diverse organisms, including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and other nonfastidious and moderately fastidious bacteria. It is not selective or differential and may be used as a base medium for supplementation when specific growth factors or antibiotics are required for particular applications.

  • 1.7% Tryptone
  • 0.3% Soytone
  • 0.25% Cerelose Anhydrous Dextrose (Glucose)
  • 0.5% Sodium chloride
  • 0.25% Potassium phosphate dibasic
  • General Bacterial Cultivation: Broad-spectrum growth medium for routine propagation of aerobic and facultative bacteria, including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis.

  • Sterility Testing (Compendial): Soybean-Casein Digest Medium for USP/EP/JP sterility tests of pharmaceuticals, medical devices, and sterile fluids.

  • Growth Promotion Testing (GPT): Quality control verification of culture media using challenge organisms such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and Aspergillus brasiliensis.

  • Sample Enrichment (Clinical, Food, and Environmental): Pre-enrichment and recovery of stressed organisms, including Salmonella, Listeria monocytogenes, and Staphylococcus aureus.

  • Blood Culture Base: Used as a base medium for aerobic blood culture systems when supplemented with blood/anticoagulants.

  • Bioburden and Contamination Monitoring: Detection and enumeration of microbial contaminants in process waters, raw materials, and finished products.

  • Culture Maintenance and Expansion: Preparation of seed cultures, growth curves, and inocula for downstream assays and fermentation.

  • Antibiotic Studies (Preparation/Recovery): Cultivation and recovery of test strains prior to susceptibility testing or potency assays.

  • Environmental Monitoring: Enrichment of microorganisms from swabs, air samplers, and surface rinses in cleanroom and facility monitoring programs.

  • Phage and Molecular Biology Workflows: Propagation of bacterial hosts for phage amplification and recovery of cells post-transformation.

  • pH: 7.30 ± 0.10
Storage Temperature
Room Temperature
Shelf Life
730 days
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