Custom Order

We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

M2109

5X Supplement EZ Minus Methionine, 200mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

250mL

Item Count

1 Bottle(s)

Price per Item

$83.00

Fill Volume

200 mL

COA
COA

M2109

5X Supplement EZ Minus Methionine, 200mL, Sterile.

Quantity

Minimum is set to 8 for this product*

scroll

Description
Formulation
Common Applications
Storage

5X Supplement EZ Minus Methionine is a 5X concentrated defined supplement for preparing synthetic dropout media lacking methionine. When combined with yeast nitrogen base and an appropriate carbon source, it provides a complete amino acid and nucleotide supplement set minus methionine for formulating methionine-omission media.

This supplement is suitable for cultivating and selecting auxotrophic Saccharomyces cerevisiae strains in methionine-dropout conditions and for maintaining plasmids or genomic integrations that rely on methionine prototrophy-based selection in defined media.

  • 5X Supplement EZ Solution minus Methionine

  • Auto-Induction of Protein Expression: Formulates methionine-free EZ auto-induction media for high-yield recombinant protein production in Escherichia coli.

  • Selenomethionine Labeling: Supports SeMet incorporation into recombinant proteins by supplementing methionine-free cultures for crystallography phasing.

  • Methionine Auxotroph Studies: Enables selection and characterization of methionine auxotrophs in E. coli and related bacteria.

  • Isotopic/Radioactive Labeling: Facilitates 13C/15N or 35S-methionine metabolic labeling by providing a methionine-free background.

  • Noncanonical Amino Acid Incorporation: Allows AHA/HPG methionine-analog labeling of nascent proteins in E. coli.

  • Expression Screening: Streamlines strain and condition screening for recombinant expression using EZ auto-induction workflows.

  • Process Scale-Up: Provides consistent methionine-free supplementation from small-scale tests to bioreactor runs in E. coli.

  • Metabolic Pathway Research: Supports studies of sulfur amino acid metabolism and methionine biosynthesis regulation in bacteria.

Storage Temperature
Frozen
Shelf Life
730 days
Shows related citations for this product
Explore related products
Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

GET YOUR COA
We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button