Yeast Two-Hybrid Screening: Selects for LEU2 and TRP1 markers (-Leucine, -Tryptophan) while supporting galactose-driven reporter activation.

Plasmid Maintenance and Selection: Maintains yeast plasmids carrying LEU2/TRP1 auxotrophic markers under dropout conditions.

GAL Promoter Induction: 2.0% D-Galactose enables induction of GAL1/10-driven gene expression in yeast.

Transformation Recovery and Selection: Supports growth and selection of transformed yeast on -Leu/-Trp media.

Replica Plating and Colony Screening: Facilitates dropout-based verification of auxotrophic markers and construct integrity.

Protein–Protein Interaction Assays: Enables interaction readouts in GAL-based yeast two-hybrid workflows.

CRISPR Editing Workflows: Provides defined selection for edited yeast strains using LEU2/TRP1 markers.

Growth and Fitness Assays: Defined nitrogen (0.5% ammonium sulfate) and phosphate buffer (0.54% disodium phosphate/0.86% monosodium phosphate) support reproducible phenotyping.

Library Construction and Screening: Supports selection and enrichment of yeast genomic or cDNA libraries under -Leu/-Trp conditions.

Diploid/Mating Selections: Enables selection of diploids and double auxotrophs using combined dropout markers.