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D5120

Diluent Solution. 1000mL, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$126.00

Fill Volume

1000 mL

D5120

Diluent Solution. 1000mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Storage

Diluent Solution is an isotonic phosphate-buffered saline-based diluent containing a protein stabilizer and a non-ionic surfactant for preparation and dilution of biological samples, calibrators, and reagents.

The buffer maintains physiological ionic strength and pH during handling, while the protein component reduces adsorption to plastics and the surfactant improves wetting and dispersion in washing and assay workflows.

  • 1.00% Bovine Serum Albumin(BSA)
  • 0.05% (v/v) Tween-20
  • 135mM Sodium Chloride
  • 2.7mM Potassium Chloride
  • 4.3mM Disodium Phosphate
  • 1.4mM Monopotassium Phosphate
  • ELISA/CLIA Sample and Standard Diluent: Stabilizes proteins with 1.00% BSA and reduces nonspecific binding with 0.05% Tween-20 in PBS (135 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4).

  • Antibody/Conjugate Diluent: Optimizes capture/detection antibody and HRP/AP conjugate performance while minimizing background.

  • Plate Blocking and Reagent Diluent: Serves as a blocking buffer and diluent on ELISA plates to prevent nonspecific adsorption.

  • Immunoassay Wash Buffer: Functions as a gentle wash to lower background via 0.05% Tween-20 while maintaining ionic strength with PBS.

  • Western Blot Antibody Diluent/Blocking: Dilutes primary/secondary antibodies and blocks membranes to reduce nonspecific binding.

  • Immunofluorescence/IHC Antibody Diluent: Stabilizes antibodies and decreases off-target binding during tissue/cell staining.

  • Lateral Flow Assay Diluent: Enhances assay consistency by reducing matrix effects and nonspecific interactions.

  • Bead-Based Multiplex Assays (e.g., Luminex): Used as a sample and reagent diluent to minimize bead aggregation and background.

  • Protein/Antibody Stabilization During Dilution: Preserves activity and prevents surface adsorption during sample prep.

  • General Diagnostic Reagent Diluent: Provides consistent ionic strength and surfactant/protein additives for robust assay performance.

Storage Temperature
Refrigerated
Shelf Life
730 days
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