Nucleic Acid Preservation (TE Buffer): Chelates Mg2+/Ca2+ to inhibit DNases/RNases, stabilizing DNA/RNA in Tris-EDTA storage buffers.

Plasmid DNA Preparation from Escherichia coli: Included in resuspension buffers to inactivate nucleases and weaken the outer membrane during alkaline lysis.

Quenching Enzymatic Reactions: Stops Mg2+-dependent enzymes (e.g., polymerases, ligases, restriction endonucleases, DNases) by chelating divalent cations.

Protein Extraction and Protease Control: Inhibits metalloproteases in cell lysis and purification buffers to protect proteins from degradation.

IMAC Resin Stripping: Removes Ni2+/Co2+ from immobilized metal affinity chromatography resins for regeneration.

Competent Cell and Transformation Workflows: Used to chelate surface-stabilizing cations, aiding permeability of Gram-negative cells like Escherichia coli.

Cell Culture Detachment (Trypsin-EDTA): Facilitates dissociation of adherent mammalian cells by chelating Ca2+/Mg2+ that mediate cell–substrate adhesion.

Bacterial Cell Lysis Support: Enhances lysozyme and detergent-mediated lysis of Gram-negative bacteria (e.g., Escherichia coli, Pseudomonas aeruginosa) by disrupting LPS stabilization.

Metal-Free Buffer Preparation: Removes trace divalent metals from reagents to control metal-dependent biochemical assays.

Tissue Decalcification: Demineralizes bone and calcified samples in histology while preserving biomolecular integrity.