Custom Order

We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

F2344

5-FOA Selective Plates. 100mm Plates, Sterile. 10 plates/sleeve.

Availability

Ships in 6 - 14 Days

Container Size

100mm

Item Count

10 Plate(s)

Price per Item

$351.00

Fill Volume

25 mL

COA
COA

F2344

5-FOA Selective Plates. 100mm Plates, Sterile. 10 plates/sleeve.

Quantity

Minimum is set to 8 for this product*

scroll

Description
Formulation
Common Applications
Storage

5-FOA Selective Plates are synthetic defined agar plates for growth of yeast and counter-selection against URA3 function. The medium is based on yeast nitrogen base without amino acids or ammonium sulfate, with glucose as the carbon source, ammonium sulfate as the inorganic nitrogen source, and a complete supplement mix to supply amino acids and bases; 5‑fluoroorotic acid enables selection of ura3 cells and loss of URA3-marked elements.

  • 0.17% Yeast Nitrogen Base w/o AA/AS
  • 0.13% AA CSM Mix
  • 2.00% Agar
  • 0.10% 5-fluorouracil-6-carboxylic acid monohydrate (5-FOA)
  • 0.50% Ammonium Sulfate
  • 2.00% Cerelose Anhydrous Dextrose (Glucose)

  • URA3 Counterselection in Yeast Genetics: Selects against URA3+ cells to isolate ura3 mutants or confirm loss of URA3 markers in Saccharomyces cerevisiae and related yeasts.

  • Marker Recycling/Pop-out Screening: Identifies successful excision of URA3 cassettes after homologous recombination or site-specific recombination steps.

  • Plasmid Shuffling: Counterselects URA3-marked plasmids to test essential gene alleles or swap functional variants.

  • CRISPR/Cas9 Editing Workflows: Enables selection/counterselection cycles using URA3 as a recyclable marker during iterative genome edits.

  • Mutation Mapping in Pyrimidine Biosynthesis: Screens for 5-FOA–resistant clones indicating mutations in URA pathway genes (e.g., URA3, URA5, URA2).

  • Yeast Two-Hybrid/Reporter Systems: Counterselects URA3-based reporters to validate interactions or eliminate background signal.

  • Genetic Stability and Loss-of-Function Assays: Monitors spontaneous loss of URA3 markers, plasmid maintenance, or recombination events.

  • Strain Construction Quality Control: Confirms correct integration/excision events during stepwise strain engineering using URA3 selection/counterselection cycles.

Storage Temperature
Refrigerated
Shelf Life
90 days
Shows related citations for this product
Explore related products
Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

GET YOUR COA
We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button