Cryopreservation of Microbial Stocks: 99.5% Glycerol is used as a cryoprotectant for long-term storage of bacteria such as Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa at −80°C.

PCR/qPCR Enhancement: 99.5% Glycerol stabilizes DNA polymerases and improves amplification of GC-rich templates.

Enzyme and Protein Stabilization: 99.5% Glycerol is included in storage buffers to prevent aggregation and loss of activity during freeze–thaw cycles.

Electrophoresis Sample Loading: 99.5% Glycerol increases sample density in DNA/RNA/protein loading dyes for reliable well loading.

Glycerol Density Gradients: 99.5% Glycerol is used to prepare gradients for ultracentrifugation of proteins and ribonucleoprotein complexes.

Restriction Digests and Ligation Reactions: 99.5% Glycerol helps maintain enzyme stability and activity in molecular biology workflows.

Carbon Source in Culture Media: 99.5% Glycerol serves as an alternative carbon source for bacteria such as Escherichia coli and Pseudomonas aeruginosa.

Cell Freezing Media: 99.5% Glycerol is used as a cryoprotectant in freezing protocols for prokaryotes and select eukaryotic cells.

Viscosity/Osmoprotection in Assays: 99.5% Glycerol reduces evaporation, adjusts viscosity, and protects sensitive enzymes during assays.

Protein Storage and Crystallography: 99.5% Glycerol is added to storage and crystallization solutions to prevent ice formation and preserve structure.