pH Adjustment and Titration: Adjusts pH of buffers, media, and reagents to alkaline targets using 1N Sodium Hydroxide for precise control.

Sterile Media Preparation: Sterile 1 mL aliquots enable aseptic pH correction of cell culture media and buffers.

Plasmid Miniprep (Alkaline Lysis): Denatures chromosomal DNA and proteins during lysis of Escherichia coli for plasmid extraction.

DNA Denaturation for Blotting: Denatures DNA in agarose gels prior to alkaline Southern blot transfer.

Chromatography CIP/Sanitization: Regenerates and sanitizes chromatography columns and systems; helps remove biofilm and endotoxins.

Equipment Decontamination: Alkaline cleaning of labware and bioprocess equipment to inactivate contaminants including Escherichia coli and Staphylococcus aureus.

Neutralization of Acids: Neutralizes acidic samples, fractions, or waste streams prior to disposal or downstream processing.

Base-Catalyzed Hydrolysis: Supports saponification and other NaOH-driven hydrolysis reactions in analytical and preparative workflows.

Endotoxin Reduction Steps: Used in alkaline treatments to reduce endotoxin burden on surfaces and resins where compatible.