Antibiotic Selection of Transformants: Isolation of chloramphenicol-resistant colonies after transformation of E. coli or Agrobacterium tumefaciens with cat-marked plasmids (12.5 µg/mL chloramphenicol).

Plasmid Maintenance: Stable propagation of cat-bearing plasmids, BACs, fosmids, or cosmids in E. coli under chloramphenicol selection.

Colony Screening and Picking: Growth of individual recombinant colonies for downstream PCR, miniprep, or sequencing workflows.

Streaking for Single Colonies: Strain purification and clonal isolation of chloramphenicol-resistant bacteria (e.g., E. coli).

CFU Enumeration: Quantification of viable chloramphenicol-resistant cells after transformation, conjugation, or electroporation.

Expression Strain Maintenance: Routine culture of protein expression strains harboring cat-selectable plasmids in E. coli.

Agrobacterium Workflows: Selection and maintenance of cat-marked vectors in Agrobacterium tumefaciens for plant transformation pipelines.

Contamination Control: Suppression of chloramphenicol-sensitive contaminants while maintaining target resistant strains.

Library Construction and Screening: Selection of genomic or cDNA library clones carrying chloramphenicol-resistant vectors.

Short-Term Storage and Transport: Plate-based preservation and shipment of chloramphenicol-resistant strains.