Routine Bacterial Culturing: Supports robust growth of nonfastidious microbes, especially E. coli, for day-to-day propagation.

Plasmid Cloning and Amplification: Used to grow transformants and expand cultures for high-yield plasmid DNA preparation.

Recombinant Protein Expression: Serves as a standard medium for expressing heterologous proteins in E. coli prior to induction and scale-up.

Transformation Recovery/Outgrowth: Provides gentle nutrient conditions for heat-shock or electroporated E. coli to recover and express antibiotic resistance markers.

Antibiotic Selection in Liquid Culture: Maintains selective pressure (e.g., ampicillin, kanamycin) to preserve plasmids during overnight growth.

Starter Cultures and Inocula: Used to generate healthy seed cultures for inoculating richer media, fermenters, or expression workflows.

Strain Maintenance and Glycerol Stocks: Grows colonies to mid/late log phase prior to long-term cryopreservation.

General Microbiology Teaching and QC: Provides a reliable, reproducible medium for lab exercises and routine quality control assays.