Auxotroph/Prototroph Selection: Defined medium (1X M9 Salts, 1.0% glucose, 1 mM thiamine, 0.2 mM MgSO₄, 0.1 mM CaCl₂, 1.5% agar) for screening nutritional mutants in E. coli.

Defined-Condition Growth Studies: Controlled colony growth and physiology of enteric bacteria (e.g., E. coli, Salmonella enterica) under minimal nutrients.

Carbon Source Utilization Assays: Assess glucose-based metabolism, catabolite repression, and pathway analysis in E. coli on minimal agar.

Genetic Selection with Metabolic Markers: Selection of recombinants using markers (e.g., his, leu, lac) by adding or omitting specific supplements.

Antibiotic Selection of Transformants: Plating of plasmid-bearing E. coli on minimal medium supplemented with appropriate antibiotics/nutrients.

Bacteriophage Assays: Plaque assays with E. coli hosts under defined nutrient conditions for phage-host interaction studies.

Stress and Starvation Experiments: Evaluate growth under nutrient limitation, adaptive responses, and fitness of mutants.

Colony Isolation and Enumeration: Obtain isolated colonies and CFU counts of minimal medium-adapted strains (e.g., E. coli, Pseudomonas aeruginosa).