C. elegans Cultivation: Routine maintenance and propagation on E. coli OP50 lawns using sterile 100 mm plates.

RNAi Feeding Assays: Gene knockdown using E. coli HT115(DE3) expressing dsRNA on NGM plates.

Genetic Screens and Mutant Selection: Scoring and isolating phenotypes in forward/reverse genetic studies.

Development, Lifespan, and Reproduction: Measuring growth rate, longevity, fecundity, and brood size under controlled conditions.

Behavioral Assays: Chemotaxis, locomotion, egg-laying, and feeding (pharyngeal pumping) on standardized media.

Host–Pathogen Interaction: Infection and survival assays using pathogen lawns such as Pseudomonas aeruginosa PA14, Serratia marcescens, and Staphylococcus aureus.

Toxicology and Drug Screening: Assessing compound effects via plate dosing or seeded-lawn delivery.

Dauer and Stress Response: Inducing and quantifying dauer formation and stress tolerance under defined conditions.

Mating and Crosses: Plate-based crosses for strain construction and inheritance studies.

Microbial Diet and Metabolism Studies: Comparing worm physiology on alternative bacterial diets (e.g., E. coli HB101, NA22) or defined communities.