Selective Isolation of Gram-Positive Cocci: Enriches for Staphylococcus aureus, Streptococcus pyogenes, and Enterococcus faecalis from mixed specimens by inhibiting Gram-negative bacilli.

Recovery of Gram-Positive Anaerobes: Supports isolation of anaerobes such as Clostridium spp. and Peptostreptococcus under anaerobic conditions while suppressing Gram-negative growth.

Suppression of Gram-Negative Overgrowth: Reduces growth of Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae in polymicrobial samples for clearer Gram-positive recovery.

Inhibition of Proteus Swarming: Limits swarming by Proteus spp. to facilitate isolation of discrete Gram-positive colonies.

Clinical Specimen Processing: Used for skin, wound, respiratory, and urogenital specimens to recover staphylococci, streptococci, and enterococci when Gram-negatives are present.

Purity and Contamination Checks: Screens Gram-positive cultures to detect and suppress Gram-negative contaminants.

Environmental and Food Testing: Isolates and enumerates Gram-positive flora in samples with high Gram-negative background.

Pre-Susceptibility Colony Isolation: Obtains isolated Gram-positive colonies for AST workflows from mixed microbial populations.