Custom Order

We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

P0239

1X PBS with 0.05% Tween 20, 0.1% BSA. 1000mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$85.00

Fill Volume

1000 mL

COA
COA

P0239

1X PBS with 0.05% Tween 20, 0.1% BSA. 1000mL, Sterile.

Quantity

Minimum is set to 8 for this product*

scroll

Description
Formulation
Common Applications
Storage

Phosphate-buffered saline containing a nonionic surfactant and a carrier protein is an isotonic wash and dilution buffer for immunoassays and general laboratory workflows. The buffer maintains physiological pH and osmolarity during sample handling and wash steps.

The surfactant promotes efficient wetting and helps disrupt weak hydrophobic interactions during washes, while the protein component provides a background matrix that reduces nonspecific adsorption of antibodies and other proteins to plastics and membranes. Applications include ELISA plate washing, Western blot wash and block steps, immunofluorescence washes, and preparation of antibody diluents.

  • 1X PBS Buffer with 0.05% Tween-20 (v/v) and 0.1% Bovine Serum Albumin (BSA)

  • Western Blotting: Blocking and wash buffer to reduce non-specific antibody binding on membranes.

  • ELISA: Plate blocking, sample/antibody diluent, and wash steps to minimize background signal.

  • Immunohistochemistry/Immunocytochemistry: Antibody diluent and wash buffer to improve specificity in tissue and cell staining.

  • Immunofluorescence Microscopy: Reduces non-specific binding and background during antibody staining.

  • Flow Cytometry: Staining wash and antibody diluent to decrease cell-surface non-specific interactions.

  • Dot Blot/Colony Blot: Membrane blocking and washing to enhance signal-to-noise.

  • Bead-Based Immunoassays: Magnetic/agarose bead blocking and washes for cleaner binding assays.

  • Surface Blocking: Pre-blocking microplates, tubes, and biosensor surfaces to prevent non-specific adsorption.

  • Antibody/Protein Dilution: Stabilizing diluent for primary/secondary antibodies and proteins.

  • Microbial Antigen Assays: Used in ELISAs and blots involving Escherichia coli or Staphylococcus aureus antigens to reduce background.

Storage Temperature
Refrigerated
Shelf Life
730 days
Shows related citations for this product
Explore related products
Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

GET YOUR COA
We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button