Bacteriophage Storage and Stabilization: Preserves phage infectivity using 50 mM Tris-HCl, 100 mM NaCl, 10 mM MgSO₄, and 0.01% gelatin to maintain pH, ionic strength, and reduce surface adsorption.

Phage Dilution and Titering: Used for serial dilutions prior to plaque assays, ensuring consistent ionic conditions for accurate titers.

Plaque Assays: Employed to resuspend plaques and prepare phage stocks for enumeration and isolation.

Elution from Agar or Filters: Facilitates elution of phage from agar plugs, soft agar overlays, and membrane filters.

Phage Lysate and Pellet Resuspension: Resuspends PEG/NaCl-precipitated or centrifuged phage and is compatible with downstream CsCl gradients.

Phage DNA Extraction Prep: Serves as a stabilizing buffer for phage particles before nuclease treatments and genome isolation.

Phage Display and Library Handling: Maintains phage library stability during amplification, storage, and panning workflows.

Transport Medium for Phage Samples: Stabilizes environmental and clinical phage samples during collection and shipment.

Transduction Workflows: Storage and handling of P1, P22, and related lysates used for generalized transduction in Escherichia coli, Salmonella enterica, and Staphylococcus aureus.

Host Range and Infection Assays: Diluent for setting MOI and preparing inocula with phages infecting Escherichia coli and Pseudomonas aeruginosa.