Cell Lysis and Membrane Disruption: Efficient lysis of bacterial (Escherichia coli, Bacillus subtilis), yeast, and mammalian cells for downstream nucleic acid and protein workflows.

Genomic DNA and RNA Extraction: Included in lysis buffers to solubilize membranes and denature proteins during phenol–chloroform or column-based purification.

Plasmid Preparation: Enhances bacterial lysis and helps clear lipid/membrane contaminants in alkaline lysis variants.

Membrane Protein Solubilization: Solubilizes and stabilizes membrane proteins prior to purification or activity assays.

Inclusion Body Processing and Refolding: Solubilizes aggregated proteins before refolding screens and optimization.

Chromatin and Nuclei Preparation: Used in lysis buffers for chromatin shearing, ChIP, and nuclei isolation.

Metagenomic DNA Isolation: Facilitates extraction from mixed microbial communities, including Gram-negative species like Pseudomonas aeruginosa.

Viral Envelope Disruption: Solubilizes envelopes to inactivate viruses and release RNA for RT-qPCR.

Ribonucleoprotein and Ribosome Studies: Employed to dissociate ribosomes and RNPs for footprinting or complex analysis.