Density Gradient Centrifugation: Preparation of sucrose gradients (e.g., 20–60%) to separate viruses, organelles, and vesicles by buoyant density.

Ultracentrifugation Cushions: Use as a 60% Sucrose Solution cushion to concentrate and purify viral vectors (AAV, lentivirus), bacteriophages, and extracellular vesicles.

Polysome and Ribosome Profiling: Isolation of ribosomes and polysomes from mammalian cells and E. coli using sucrose gradients.

Membrane and Organelle Fractionation: Separation of plasma membranes, mitochondria, nuclei, and other subcellular components via rate-zonal centrifugation.

Protoplast/Spheroplast Stabilization: Osmotic stabilization during formation and handling of bacterial spheroplasts (e.g., E. coli, Bacillus subtilis) and yeast protoplasts.

Virus Purification and Concentration: Purification of viruses and bacteriophages from complex mixtures using step or continuous sucrose gradients.

Extracellular Vesicle Isolation: Rate-zonal separation of exosomes and microvesicles from cell culture supernatants and biofluids.

Endotoxin-Sensitive Workflows: Suitable for applications requiring low endotoxin levels, including cell culture, vaccine research, and bioprocessing.